About

Biology as an
engineering discipline.

Konstantinos Kariotis

I am now based in Copenhagen at the Novo Nordisk Foundation Center for Protein Design (CPD), University of Copenhagen, where I am beginning an integrated MSc–PhD trajectory focused on de novo protein design.

My research interests centre on designing proteins with functions that do not exist in nature, particularly through the combination of computational protein design, structural biology, synthetic biology, and experimental validation.

My current work at CPD includes projects involving de novo β-sheet and β-sandwich design, and the design of proteins capable of interacting selectively with persistent environmental contaminants such as PFAS. The PFAS work explores de novo proteins for selective binding, sensing, sequestration, and potentially degradation of fluorinated compounds. My work draws on generative protein design, molecular modelling, sequence design, structural prediction, and experimental protein engineering.

I am particularly interested in the idea of treating biology as an engineering discipline: understanding the design principles encoded in natural proteins, and using those principles to create new molecular systems. I enjoy working across the boundary between computation and experiment, using computational methods not simply to analyse biological systems, but to design and build them.

Research interests

  • 01

    De novo protein design

    Designing proteins with functions that do not exist in nature — new folds, new activities, new molecular systems built from first principles.

  • 02

    β-sheet and β-sandwich design

    Current work on de novo β-sheet and β-sandwich architectures, where computational design and structural biology meet experimental validation.

  • 03

    PFAS-binding proteins

    Engineering proteins for selective binding, sensing, sequestration, and potentially degradation of persistent fluorinated compounds.

  • 04

    Biology as an engineering discipline

    Understanding the design principles encoded in natural proteins, and using those principles to build new molecular systems.

Education

  • University of Copenhagen

    University of Copenhagen

    Integrated MSc–PhD, Protein Design

  • University of Bristol

    University of Bristol

    BSc Biochemistry

  • Anatolia College

    Anatolia College

    International Baccalaureate Diploma Programme

Skills & methods

  • Computational protein design
  • Generative protein design
  • Molecular modelling
  • Sequence design
  • Structural prediction
  • Structural biology
  • Synthetic biology
  • Experimental protein engineering
  • Protein expression & purification
  • Molecular cloning
  • CRISPR-associated transposons
  • Python, R, MATLAB
  • AlphaFold2 · PyMOL